Bacillus sphaericus is defined as a mesophilic, aerobic Gram-positive bacterium found in soil and aquatic environments, distinguishable by its spherical spores located at one end of a swollen sporangium. It has garnered interest for its potential use as a mosquito larvicide, with various strains identified based on serotypes and DNA homologies.

The use of insecticides for mosquito vector control in recent decades has caused environmental pollution of aqueous ecosystems and resulted in insecticide resistance in many mosquito species. Biological means to control vectors based on entomopathogenic bacteria, Bacillus thuringiensis Berliner subsp. israelensis and Bacillus sphaericus Meyer and Neide, has been studied for more than 20 years. The larvicidal substances of these preparations are based on δ-endotoxin proteins accumulated as parasporal crystals produced by the bacterial cells during the sporulation phase. These biological preparations have the advantages of high specificity (selectivity) resulting in low negative impact to the environment. 

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